Terminator

Part:BBa_K3384311:Design

Designed by: Yiqing Zhang   Group: iGEM20_NJTech_China   (2020-10-18)


CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Terminator is a special DNA sequence that is located downstream of the coding region of a gene and can give an RNAP transcription termination signal. We amplified it from the pCAS(#60847).


Source

The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as a template.

References